Preparation for Physical Agents of Microbial Control
Lab Exercise

Label 4 tubes of TS broth with the following: ER, BR, EF, BF.  Inoculate the "E" tubes with E. coli, and the "B" tubes with B. subtilis.  Tubes ER and BR will be placed in the refrigerator until the next lab period.  Tubes EF and BF will be placed in the freezer until the next lab period.

Label 2 empty petri plates ED and BD.  With a sterile cotton swab, smear the "E" plate with E. coli broth, and the "B" plate with B. subtilis broth.  Tape the plates closed and place them in the designated drawer until the next lab period.