Introduction to Medical Microbiology
Exam #1 Study Guide
Introduction/History (see chapter questions 1,2,5,6,7,8,10,13,14,15)
1. Be able to define microbiology, immunology, epidemiology, biotechnology, and genetic engineering
2. Be able to list the examples of microorganisms and define pathogens and viruses
3. Be able to identify the people of historical significance and what each oneÕs contribution to microbiology was.
4. Know the difference between hypothesis and theory and define Taxonomy, Identification, Classification, and Nomenclature.
5. Know the difference between Species and Domain and know the three Domains in which organisms are classified.
6. Know the 3 rules of binomial system of nomenclature and who generated it.
Chemistry of Biology/Tools of the Lab (see 1,2,7,8,9,13,15 in Ch.2 and 1,2,14,15 in Ch.3)
1. Define atom and know the difference between proton, neutron, and electron.
2. Know the basics of pH, organic and inorganic chemicals, proteins, carbohydrates, nucleic acids, and lipids, including examples if given
3. Know the the Five IÕs used to study microorganisms and the basics of each, including the definition of a colony, 2 methods used for isolation, and 3 types of cultures for inspection.
4. Know how media is classified based on physical state (3 types) and chemical content (2 types plus definitions).
5. Know the differences between differential, enriched, selective, and general purpose media.
6. Know the 2 types of microscopes and the basics of magnification, resolution, and contrast.
7. Know the basic characteristics of the 5 different types of microscopes (bright-field, dark-field, etc.) and any special uses of each.
8. Know the purpose of a wet mount and the basic characteristics of positive stains (simple, differential, special) and negative stains.
Prokaryotic Cell Structure/Classification of Prokaryotes (see 2,3,4,5,7,8,10,11,14)
1. Know the characteristics of prokaryotic cells
2. Know the function of the flagella and its 3 components and their descriptions.
3. Be able to describe the 4 types of flagella arrangements
4. Know definition of chemotaxis, the difference between positive and negative chemotaxis, and be able to describe a run and tumble.
5. Be able to describe the structure and/or functions of fimbriae, pilus, glycocalyx, cell wall, cytoplasmic membrane, chromosome, plasmid, ribosomes, and storage granules.
6. Know the main structural characteristics of Gram(+) and Gram (-) cell walls
7. Know the characteristics of endospores and the purpose and process of sporulation
8. Know descriptions of the basic bacterial shapes (bacillus, coccus, etc.).
9. Know the differences between Bacteria and Archaea.
10. Be able to identify examples of gram (-) bacilli, gram (-) curved bacilli, gram (-) cocci, gram (+) bacilli, gram (+) cocci, and acid-fast bacilli.
11. Be able to define and give examples of obligate intracellular parasites, Enterobacteriaceae, spore-forming bacilli, and spirochetes.
12. Know any specific characteristics of certain bacterial species if listed
Eukaryotic Organisms (see 1,3,4,5,6,13)
1. Know the characteristics of eukaryotic cells
2. Know the basic characteristics of fungi, including their methods of reproduction
3. Know 3 roles of fungi in medicine and industry, including the aspects of mycoses and the 4 specific examples of fungi given.
4. Know the basic characteristics of algae, the different types of reproduction, and the diseases they cause.
5. Know the basic characteristics of protozoans and be able to identify examples of protozoans based on their mode of locomotion.
6. Know the basic characteristics of helminthes and the general differences between Nematodes, Cestodes, and Trematodes.
7. Be able to match each helminth with the name of the disease it causes and know the basic descriptions of the diseases or organisms if indicated (especially method of transmission).